Bacillus vallismortis strain BZ1 16S ribosomal RNA gene, partial sequence
(1–4)LOCUS KJ642605 1496 bp DNA
linear BCT 21-JUN-2014
DEFINITION Bacillus vallismortis strain BZ1 16S
ribosomal RNA gene, partial
sequence.
ACCESSION KJ642605
VERSION KJ642605.1
KEYWORDS .
SOURCE Bacillus vallismortis
ORGANISM
Bacillus vallismortis
Bacteria; Firmicutes; Bacilli;
Bacillales; Bacillaceae; Bacillus.
REFERENCE 1
(bases 1 to 1496)
AUTHORS
Babuselvam,M., Abideen,S., David,B. and Riswana Barveen,J.H.
TITLE
Identification of fish gut microbes for antagonistic activity
against human pathogens
JOURNAL
Unpublished
REFERENCE 2
(bases 1 to 1496)
AUTHORS
Babuselvam,M., Abideen,S., David,B. and Riswana Barveen,J.H.
TITLE
Direct Submission
JOURNAL
Submitted (28-MAR-2014) P. G. and Research Department of Zoology,
Dr. Zakir Husain College,
Ilayangudi, Sivagangai, Tamil Nadu
630702, India
Sequencing Technology :: Sanger
dideoxy sequencing
##Assembly-Data-END##
source 1..1496
/organism="Bacillus
vallismortis"
/mol_type="genomic
DNA"
/strain="BZ1"
/db_xref="taxon:72361"
/country="India"
rRNA
<1..>1496
/product="16S
ribosomal RNA"
ORIGIN
61 gatgttagcg gcggacgggt gagtaacacg
tgggtaacct gcctgtaaga ctgggataac
121 tccgggaaac cggggctaat accggatggt
tgtttgaacc gcatggttca gacataaaag
181 gtggcttcgg ctaccactta cagatggacc
cgcggcgcat tagctagttg gtgaggtaac
241 ggctcaccaa ggcaacgatg cgtagccgac
ctgagagggt gatcggccac actgggactg
301 agacacggcc cagactccta cgggaggcag
cagtagggaa tcttccgcaa tggacgaaag
361 tctgacggag caacgccgcg tgagtgatga
aggttttcgg atcgtaaagc tctgttgtta
421 gggaagaaca agtgccgttc aaatagggcg
gcaccttgac ggtacctaac cagaaagcca
481 cggctaacta cgtgccagca gccgcggtaa
tacgtaggtg gcaagcgttg tccggaatta
541 ttgggcgtaa agggctcgca ggcggtttct
taagtctgat gtgaaagccc ccggctcaac
601 cggggagggt cattggaaac tggggaactt
gagtgcagaa gaggagagtg gaattccacg
661 tgtagcggtg aaatgcgtag agatgtggag
gaacaccagt ggcgaaggcg actctctggt
721 ctgtaactga cgctgaggag cgaaagcgtg
gggagcgaac aggattagat accctggtag
781 tccacgccgt aaacgatgag tgctaagtgt
tagggggttt ccgcccctta gtgctgcagc
841 taacgcatta agcactccgc ctggggagta
cggtcgcaag actgaaactc aaaggaattg
901 acgggggccc gcacaagcgg tggagcatgt
ggtttaattc gaagcaacgc gaagaacctt
961 accaggtctt gacatcctct gacaatccta
gagataggac gtccccttcg ggggcagagt
1021 gacaggtggt gcatggttgt cgtcagctcg
tgtcgtgaga tgttgggtta agtcccgcaa
1081 cgagcgcaac ccttgatctt agttgccagc
attcagttgg gcactctaag gtgactgccg
1141 gtgacaaacc ggaggaaggt ggggatgacg
tcaaatcatc atgcccctta tgacctgggc
1201 tacacacgtg ctacaatgga cagaacaaag
ggcagcgaaa ccgcgaggtt aagccaatcc
1261 cacaaatctg ttctcagttc ggatcgcagt
ctgcaactcg actgcgtgaa gctggaatcg
1321 ctagtaatcg cggatcagca tgccgcggtg
aatacgttcc cgggccttgt acacaccgcc
1381 cgtcacacca cgagagtttg taacacccga
agtcggtgag gtaacctttt aggagccagc
1441 cgccgaaggt gggacagatg attgggggtg aagtcgtaac
aaggtagccg tatcgg
The
species Bacillus
anthracis, Bacillus cereus, Bacillus mycoides, and Bacillus thuringiensis were originally described on the basis of their habitats,
their pathogenicity for
mammals or
insects, and their morphological and physiological characteristics. However, the
taxonomic interrelationships
of these
species are equivocal. All four species share many
phenotypic properties, and several workers have questioned their status as
separate species (6, 11, 12).
DNA-DNA
hybridization studies on strains of B. anthracis, B. cereus, and B. thuringiensis have also shown that these organisms share relatively high
levels of chromosomal base
sequence
similarity (7, 10, 13). However, inconsistencies in reported levels of DNA
relatedness make it difficult to draw firm conclusions
regarding species differentiation within this group
of organisms
Five Bacillus strains isolated from Death
Valley soil were shown to belong to a previously unidentified species, for
which we propose the name Bacillus vallismortis. The type strain is
strain DV1-F-3 (= NRRLB-14890).
On the basis of previously published restriction digestion data, B.
vallismortis is most closely related to Bacillus subtilis. At this
time B. vallismortis can be distinguished from B. subtilis only
by differences in whole-cell fatty acid compositions, DNA sequences, and levels
of reassociation of genomic DNA.
Efficacy of plant growth promoting rhizobacteria
(PGPR) Bacillus vallismortis strain EXTN-1 has been proved in eliciting
induced systemic resistance (ISR) in several crops. B. vallismortis strain EXTN-1, used in the present study has been demonstrated to induce systemic resistance against
multiple pathogens in various
crops.
With
these considerations in mind, work was started to produce a system for the
ready and rapid identification of Bacillus strains. A study of a collection
of 600 strains, of which 91 were unnamed, using 119 API and 20 supplementary
tests, has been carried out (Logan & Berkeley,1981).
A development of this work (written later but published earlier), based on the
80 tests showing greatest reproducibility and discriminatory value, was
described by Logan (1980). Despite considerable between-strain variation in
many species this study showed that the majority of taxa are quite distinct.
API materials used in these trials included the 20E, 50E (bothdesigned for identification
of members of the Enterobacteriaceae), API ZYM and four
other,non-commercially available, enzyme test strips (Logan, 1980). The 50E
has now been superseded
by the 50CH.
Bacterial strains, antiserum, plasmids, and growth
conditions. Escherichia coli strains for DNA manipulation were routinely
cultured in LB medium (Miller, 1992) with aeration. B. cereus strains listed in
Table 1 were grown in BHI broth.
Dapus
Park K, Paul D, Ryu KR, Kim EY,
Kim YK. Bacillus vallismortis strain EXTN-1 mediated systemic resistance
against potato virus Y and X in the field. Plant Pathol J. 2006;22(4):360–3.
Logan
N a, Berkeley RC. Identification of Bacillus strains using the API system. J
Gen Microbiol. 1984;130(7):1871–82.
Ash C, Farrow JAE, Dorsch M,
Stackebrandt E, Collins MD. Comparative Analysis of Bacillus-Anthracis,
Bacillus-Cereus, and Related Species on the Basis of Reverse Transcriptase
Sequencing of 16S rRNA. Int J Syst Bact. 1991;41:343–6.
. Ivanova E, Vysotskii M.
Characterization of Bacillus strains of marine origin. Int … [Internet]. 2010;(January). Available from:
http://130.206.88.107/index.php/IM/article/viewFile/4c457c06dd55b.002/9223
Agata N, Ohta M, Arakawa Y, Mori
M. The bceT gene of Bacillus cereus encodes an enterotoxic protein.
Microbiology. 1995;141(4):983–8.
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